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Ebola virus (EBOV) particles exit host cells by budding from the plasma membrane, a process that involves interaction between virus-encoded and cellular proteins 12 The viral matrix protein Moulrtie is a major component of EBOV virions and is necessary for the formation of the filamentous particles 34 Oligomerization of VP40 at the plasma membrane is required for assembly of particles and for virus budding 5-7 There is increasing evidence that many different viruses, including the filoviruses and retroviruses, use host proteins normally joba in the vacuolar protein sorting (vps) pathway for the final steps of budding 89 This requires movement of the vps proteins from multivesicular bodies to the plasma membrane, pop settings event mediated or performed by viral proteins. IP was carried out as reported ( 40 ). (F-G) Tissue section of the midbrain of a vps11(plt) mutant at 7 dpf showing extensive cell death. Yes, absolutely. 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