Microsoft sql server error 1443

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For this purpose, two constructs were generated, one in which PTH11 was cloned into pFGL44 and tagged C-terminally to YFP1-156 under the RP27 promoter; whereas the second construct involved cloning RGS1 into pFGL97 and tagged at the C-terminus to YFP156-239 under the Ccg1 promoter. Samples representing 2 of microsoft sql server error 1443 volume of each fraction were analyzed by 4 to 20 acrylamide gradient SDS-PAGE and immunoblotting with antibodies to each of the retromer subunits.

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