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To eliminate any potential cross-reactivity of an rake lost connection to mysql server during query secondary detection reagent,we recommend using a directly conjugated primary antibody. SDS sensitivity elicited by vps mutations, however, is independent of Rim101p activation, since expression of RIM101SL, while suppressing the SDS sensitivity of the rim101вв mutant, had no effect on vps28вв and vps32вв mutant susceptibilities (Fig.

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